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High-speed 4D fluorescence light field tomography of whole freely moving organisms

High-speed 4D fluorescence light field tomography of whole freely moving organisms

We introduce reflective Fourier light field computed tomography (ReFLeCT), a snapshot volumetric fluorescence imaging system that captures volumetric videos of freely moving organisms at 120 volumes per second.

2025-05-05
Optica (2025)
Multi-Camera Array3D ImagingLight Field Tomography
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Rapid 3D Imaging at Cellular Resolution for Digital Cytopathology with a Multi-Camera Array Scanner (MCAS)

Rapid 3D Imaging at Cellular Resolution for Digital Cytopathology with a Multi-Camera Array Scanner (MCAS)

The Multi-Camera Array Scanner (MCAS) rapidly digitizes large 3D cytology samples with unparalleled resolution and machine learning integration for efficient and accurate pathology analysis.

2024
npj imaging (2024)
Multi-Camera ArrayPathologyMachine Learning
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Computational 3D Topographic Microscopy from Terabytes of Data Per Sample

Computational 3D Topographic Microscopy from Terabytes of Data Per Sample

STARCAM, our 54-camera 3D microscope, uses a self-supervised neural network for high-resolution gigapixel imaging, supporting diverse applications.

2024
Journal of Big Data (2024)
Multi-Camera Array3D Imaging
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Tensorial Tomographic Fourier Ptychography with Applications to Muscle Tissue Imaging

Tensorial Tomographic Fourier Ptychography with Applications to Muscle Tissue Imaging

Our new T2oFu tomographic microscopy extends Fourier ptychography for 3D polarimetric tissue imaging, leveraging vectorial light.

2024
Advanced Photonics (2024)
Fourier PtychographyPolarization3D Imaging
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Review on Digital Staining in Optical Microscopy using Deep Learning

Review on Digital Staining in Optical Microscopy using Deep Learning

This review covers digital staining, a deep learning method translating optical to biochemical contrast. It analyzes the state-of-the-art, identifies challenges, and postulates future applications.

2023
PhotoniX (2023)
PathologyMachine Learning
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scatterBrains: an Open Database of Human Head Models and Companion Optode Locations for Realistic Monte Carlo Photon Simulations

scatterBrains: an Open Database of Human Head Models and Companion Optode Locations for Realistic Monte Carlo Photon Simulations

Our new variable-angle illumination microscopy extends Fourier ptychography to acquire high-resolution, large field-of-view complex polarimetric data, accounting for light's vectorial nature.

2023
Journal of Biomedical Optics (2023)
Diffuse OpticsMonte CarloSimulation
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Imaging Dynamics Beneath Turbid Media via Parallelized Single-Photon Detection

Imaging Dynamics Beneath Turbid Media via Parallelized Single-Photon Detection

We use a SPAD array camera and deep neural network process speckle fluctuations to reconstruct deep tissue dynamics and monitor internal flow in phantoms."

2022
Advanced Science (2022)
Diffuse Optics
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Quantitative Jones Matrix Imaging Using Vectorial Fourier Ptychography

Quantitative Jones Matrix Imaging Using Vectorial Fourier Ptychography

Our new variable-angle illumination microscopy extends Fourier ptychography to acquire high-resolution, large field-of-view complex polarimetric data, accounting for light's vectorial nature.

2022
Biomedical Optics Express (2022)
Fourier PtychographyPolarization
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Increasing a Microscope’s Effective Field of View via Overlapped Imaging and Deep Learning Classification

Increasing a Microscope’s Effective Field of View via Overlapped Imaging and Deep Learning Classification

Multi-lens microscopy efficiently analyzes specimens using overlapping sensor views. Its software accurately detects malaria and WBC counting features.

2022
Optics Express (2022)
Learned SensingMachine LearningPathology
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Physics-Enhanced Machine Learning for Virtual Fluorescence Microscopy

Physics-Enhanced Machine Learning for Virtual Fluorescence Microscopy

Integrating an illumination model into deep CNNs enables learning task-specific LED patterns, significantly improving fluorescence image inference from unstained microscopy.

2021
ICCV (2021)
Learned SensingMachine Learning
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Quantized Fourier Ptychography with Binary Images from SPAD Cameras

Quantized Fourier Ptychography with Binary Images from SPAD Cameras

We study the feasibility of implementing Fourier ptychography (FP) with SPAD array cameras to reconstruct an image with higher resolution and larger dynamic range from acquired binary intensity measurements.

2021
Photonics Research (2021)
Fourier Ptychography
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Deep Optical Blood Analysis: COVID-19 Detection as a Case Study in Next Generation Blood Screening

Deep Optical Blood Analysis: COVID-19 Detection as a Case Study in Next Generation Blood Screening

A novel machine learning method using multiple instance learning to analyze peripheral blood smears, aiming to understand COVID-19's poorly understood morphological impact on various blood cell types.

2021
medRxiv (2021)
PathologyMachine Learning
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Fast and Sensitive Diffuse Correlation Spectroscopy with Highly Parallelized Single Photon Detection

Fast and Sensitive Diffuse Correlation Spectroscopy with Highly Parallelized Single Photon Detection

A high-sensitivity DCS system using a SPAD array can detect small deep tissue perturbations, measure human pulse with high fidelity, and identify physiological changes in the prefrontal cortex.

2020
APL Photonics (2020)
Diffuse Optics
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Diffraction Tomography with a Deep Image Prior

Diffraction Tomography with a Deep Image Prior

Deep Prior Diffraction Tomography (DP-DT) is a novel technique that reconstructs high-resolution 3D refractive index maps of thick biological samples from low-resolution images taken with varying illumination.

2020
Optics Express (2020)
Fourier Ptychography3D Imaging
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Multi-Element Microscope Optimization by a Learned Sensing Network with Composite Physical Layers

Multi-Element Microscope Optimization by a Learned Sensing Network with Composite Physical Layers

In this work, we investigate an approach to jointly optimize multiple microscope settings, together with a classification network, for improved performance with such automated tasks.

2020
Optics Letters (2020)
Learned SensingMachine LearningPathology
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Towards an Intelligent Microscope: Adaptively Learned Illumination for Optimal Sample Classification

Towards an Intelligent Microscope: Adaptively Learned Illumination for Optimal Sample Classification

Using reinforcement learning to discover the best way to illuminate microscope samples for faster, more accurate classification.

2020
ICASSP (2020)
Learned SensingMachine LearningPathology
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Learned Sensing: Jointly Optimized Microscope Hardware for Accurate Image Classification

Learned Sensing: Jointly Optimized Microscope Hardware for Accurate Image Classification

A method to enhance automated image classification speed and accuracy by co-optimizing microscope illumination and a deep neural network classification pipeline.

2019
Biomedical Optics Express (2019)
Learned SensingMachine LearningPathology
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