Multi Element microscope optimization

Jul 28, 2020. | By: Kanghyun Kim, Pavan Chandra Konda, Colin L.Cooke, Ron Appel and Roarke Horstmeyer.

Standard microscopes offer a variety of settings to help improve the visibility of different specimens to the end microscope user. Increasingly, however, digital microscopes are used to capture images for automated interpretation by computer algorithms (e.g., for feature classification, detection or segmentation), often without any human involvement. In this work, we investigate an approach to jointly optimize multiple microscope settings, together with a classification network, for improved performance with such automated tasks. We explore the interplay between optimization of programmable illumination and pupil transmission, using experimentally imaged blood smears for automated malaria parasite detection, to show that multi-element “learned sensing” outperforms its single-element counterpart. While not necessarily ideal for human interpretation, the network’s resulting low-resolution microscope images (20X-comparable) offer a machine learning network sufficient contrast to match the classification performance of corresponding high-resolution imagery (100X-comparable), pointing a path towards accurate automation over large fields-of-view.

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This page is an educational and research resource of the Computational Optics Lab at Duke University, with the goal of providing an open platform to share research at the intersection of deep learning and imaging system design.

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Computational Optics Lab
Duke University
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