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Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS)

Kanghyun Kim1, Amey Chaware1, Clare B. Cook1, Shiqi Xu1, Monica Abdelmalak2, Colin Cooke3, Kevin C. Zhou1,4, Mark Harfouche5, Paul Reamey5, Veton Saliu5, Jed Doman5, Clay Dugo5, Gregor Horstmeyer2, Richard Davis2, Ian Taylor-Cho2, Wen-Chi Foo2, Lucas Kreiss1, Xiaoyin Sara Jiang2, Roarke Horstmeyer1,2 1Department of Biomedical Engineering, Duke University, Durham, 27708, NC, USA. 2Department of Pathology, Duke …

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Computational 3D topographic microscopy from terabytes of data per sample

Kevin C. Zhou1,2,4, Mark Harfouche2, Maxwell Zheng2, Joakim Jönsson1, Kyung Chul Lee1,3, Ron Appel2, Paul Reamey2, Thomas Doman2, Veton Saliu2, Gregor Horstmeyer2, Seung Ah Lee3, Roarke Horstmeyer1,2 1Department of Biomedical Engineering, Duke University, Durham NC, USA. 2Ramona Optics Inc., 1000 W Main St., Durham, NC 27701, USA. 3School of Electrical and Electronic Engineering, Yonsei University, …

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scatterBrains: an open database of human head models and companion optode locations for realistic Monte Carlo photon simulations

scatterBrains: an open database of human head models and companion optode locations for realistic Monte Carlo photon simulations Melissa M. Wu1,2, Roarke Horstmeyer1, Stefan A. Carp2 1Department of Biomedical Engineering, Duke University, Durham NC, USA. 2 Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, USA. Link to paper and code Abstract Monte Carlo (MC) …

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Imaging Dynamics Beneath Turbid Media via Parallelized Single-Photon Detection

Link to paper Summary ________________________________________ Noninvasive optical imaging through dynamic scattering media has numerous important biomedical applications but still remains a challenging task. While standard diffuse imaging methods measure optical absorption or fluorescent emission, it is also well-established that the temporal correlation of scattered coherent light diffuses through tissue much like optical intensity. Few works …

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Tensorial tomographic Fourier ptychography with applications to muscle tissue imaging

Link to paper Summary ________________________________________ We report tensorial tomographic Fourier ptychography (T2oFu), a nonscanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier ptychography, a quantitative phase imaging technique, T2oFu additionally highlights the vectorial nature of light. The imaging setup consists of a standard microscope …

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Review on Digital staining in Optical Microscopy using Deep Learning

Our review on Digital staining in optical microscopy using deep learning ________________________________________ Lucas Kreiss1,2, Shaowei Jiang3 , Xiang Li4, Shiqi Xu1, Kevin C. Zhou1,5, Kyung Chul Lee1,6, Alexander Mühlberg2, Kanghyun Kim1, Amey Chaware1, Michael Ando7, Laura Barisoni8, Seung Ah Lee6, Guoan Zheng3, Kyle J. Lafata4, Oliver Friedrich 2 and Roarke Horstmeyer1 1 Department of Biomedical …

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Quantitative Jones matrix imaging using vectorial Fourier ptychography

Quantitative Jones matrix imaging using vectorial Fourier ptychography Biomedical Optics Express(2022) Xiang Dai1, Shiqi Xu1, Xi Yang1, Kevin C. Zhou1, Carolyn Glass2, Pavan Chandra Konda1, Roarke W. Horstmeyer1† 1 Department of Biomedical Engineering, Duke University, Durham, NC, USA, 27708 2 Department of Pathology, Duke University, Durham, NC, USA, 27708 Link to paper Abstract This paper …

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Quantitative Jones matrix imaging using vectorial Fourier ptychography

Quantitative Jones matrix imaging using vectorial Fourier ptychography Biomedical Optics Express(2022) Xiang Dai1, Shiqi Xu1, Xi Yang1, Kevin C. Zhou1, Carolyn Glass2, Pavan Chandra Konda1, Roarke W. Horstmeyer1† 1 Department of Biomedical Engineering, Duke University, Durham, NC, USA, 27708 2 Department of Pathology, Duke University, Durham, NC, USA, 27708 Link to paper Abstract This paper …

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Vectorial Fourier ptychography

Quantitative Jones matrix imaging using vectorial Fourier ptychography Biomedical Optics Express (2022) Xiang Dai1, Shiqi Xu1, Xi Yang1, Kevin C. Zhou1, Carolyn Glass2, Pavan Chandra Konda1, Roarke W. Horstmeyer1† 1 Department of Biomedical Engineering, Duke University, Durham, NC, USA, 27708 2 Department of Pathology, Duke University, Durham, NC, USA, 27708 Link to paper Abstract This …

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Quantized FP with binary images from SPAD Cameras

Quantized Fourier ptychography with binary images from SPAD cameras Paper Published in Photonics Research(2021) Xi Yang,1,* Pavan Chandra Konda,1 Shiqi Xu,1 Liheng Bian,2 and Roarke Horstmeyer1,3,4 1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA 2School of Information and Electronics, Beijing Institute of Technology, Beijing 100081, China 3Department of Physics, Duke University, Durham, North …

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